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| Yilmaz-Dikmen,B; Dikmen,S. |
| The present study was carried out to determine the sex of the fertilized white layer eggs with using morphological measurements. A total of 300 Super Nick White Layer (54 wks of age) eggs were incubated and sexed in the end of incubation period. Before incubation egg length, width and weight were measured. The egg volume and shape index were estimated with using these measurements for each egg. The effect of egg weight and replicate number was not significant on the sex of the hatching chick (p > 0.05). The effects of egg shape index (p = 0.001), egg length (p = 0.0018), egg width (p < 0.01) and volume (p = 0.004) of the egg had significant effect on the sex of hatching chick. According to the results of the current study, morphological... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Morphometric measurements; Pre-incubation; Sexing; Layer. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000300006 |
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| Malvasio, Adriana; Universidade Federal do Tocantins - UFT; Nascimento-Rocha, Josefa Moreira do; Universidade Federal do Tocantins - UFT; Santos, Helcileia Dias; Universidade Federal do Tocantins - UFT; Ataídes, Adson Gomes de; Universidade Federal do Tocantins - UFT; Portelinha, Thiago Costa Gonçalves; Universidade Federal do Tocantins - UFT. |
| P. expansa e P. unifilis. Todos os espécimes utilizados neste trabalho foram procedentes do rio Javaés, entorno do Parque Nacional do Araguaia, Estado do Tocantins, Brasil. Após estudo sob estereomicroscópio, foram coletadas as gônadas para o procedimento histológico de rotina. Foram analisadas 187 amostras de P. expansa, das quais 81,2% foram identificadas como fêmeas e 18,7% como machos; e 98 de P. unifilis, das quais 31,6% eram fêmeas e 68,4% machos. Não foram verificadas diferenças microscópicas relevantes na sexagem entre estas espécies. Os critérios utilizados no diagnóstico microscópico foram principalmente a morfologia das gônadas, a presença do apêndice no oviduto remanescente, a morfologia do oviduto e as características das células de... |
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Palavras-chave: 2.04.00.00-4 tartarugas; Sexagem; Ovário; Testículo; Microscopia; Tocantins 2.04.00.00-4 turtles; Sexing; Ovary; Testicle; Microscopy; Tocantins. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7257 |
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| OI, Maya; YAMADA, Keisuke; HAYAKAWA, Hiroyuki; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Effective preselection of sex has been accomplished in several species of livestock and also in humans using the flow cytometric sperm sorting method. A guaranteed high sorting accuracy is a key prerequisite for the widespread use of sperm sexing. The standard validation method is flow cytometric remeasurement of the DNA content of the sexed sperm. Since this method relies on the same instrument that produced the original sperm separation, it is not truly independent. Therefore, to be able to specifically produce either male or female offspring in the dog, we developed a method of direct visualization of sex chromosomes in a single sperm using fluorescence in situ hybridization (FISH) as a validation method. Denaturation of canine spermatozoa by immersion... |
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Palavras-chave: Dog; Fluorescence in situ hybridization (FISH); Sexing; Sperm. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3858 |
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| Lemos,Daniela Cristina; Rios,Álvaro Fabrício Lopes; Caetano,Lisandra Cristina; Lôbo,Raysildo Barbosa; Vila,Reginaldo Aparecido; Martelli,Lúcia; Takeuchi,Paula Lumy; Ramos,Ester Silveira. |
| The Y-encoded, testis-specific protein (TSPY) is a Y-specific gene. The copy numbers of TSPY range from 20 to 60 in men and up to 200 in bulls. In this study, we examined the possibility of using the TSPY gene to sex cattle. DNA from blood samples of 100 Nelore cattle (50 males and 50 females) from the Nelore Cattle Breeding Program (PMGRN) was screened for TSPY by PCR using TSPY-specific primers. The assay was highly specific since all male samples were TSPY-positive and all female samples were negative. Positive results were also obtained at low DNA concentrations (less than 1 rhog/muL). These results showed that TSPY was a good male-specific marker, the usefulness of which was enhanced by the high copy number of the gene. This is the first report to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Cattle; PCR; Sexing; TSPY. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100020 |
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